abstract：:The evasion of apoptosis has been linked to the development of cancer. A recent study of a small number of gastric and colorectal cancers found that the BAK gene, which encodes a pro-apoptotic protein, contained somatic mutations in approximately 17% of the samples analyzed. To investigate the precise frequency of BAK...

journal_title：International journal of molecular medicine

authors： Sakamoto I,Yamada T,Ohwada S,Koyama T,Nakano T,Okabe T,Hamada K,Kawate S,Takeyoshi I,Iino Y,Morishita Y

更新日期：2004-01-01 00:00:00

abstract：:Antitumor vaccination therapy approaches using naked plasmid DNA or recombinant viruses encoding tumor-associated antigens are currently in development. In the present study, we examined the therapeutic efficacy of vaccination using the mouse alpha-fetoprotein (AFP) gene in mouse hepatocellular carcinoma (HCC) cells. ...

journal_title：International journal of molecular medicine

authors： Saeki A,Nakao K,Nagayama Y,Yanagi K,Matsumoto K,Hayashi T,Ishikawa H,Hamasaki K,Ishii N,Eguchi K

更新日期：2004-01-01 00:00:00

abstract：:Recently we have shown the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various solid tumour and leukaemia cell lines as well as primary cultured bone marrow cells isolated from patients with acute and chronic myelogenous leukaemia. We further studied whether the growth inhibitory effect of ...

journal_title：International journal of molecular medicine

authors： Chui CH,Tang JC,Lau FY,Teo IT,Yau MY,Wong RS,Cheng GY,Ho SK,Leung TW,Hui KS,Wong MM,Fatima S,Cheng CH,Cheung F,Tan WQ,Chow LM,Guo D,Chan AS

更新日期：2004-01-01 00:00:00

abstract：:Trypsinogen/trypsin is one of the major serine proteases and is produced by pancreatic acinar cells. Tumor-associated trypsinogen (TAT) has been reported to be produced by several cancer cell lines. The biological roles and activation mechanisms of both TAT and pancreatic acinar trypsinogen (PAT) have not been elucida...

journal_title：International journal of molecular medicine

authors： Uchima Y,Sawada T,Nishihara T,Umekawa T,Ohira M,Ishikawa T,Nishino H,Hirakawa K

更新日期：2003-12-01 00:00:00

abstract：:Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder characterized by microthrombocytopenia, eczema, immunodeficiency, and susceptibility to lymphoid malignancy. Loss-of-function mutations in WAS gene have been identified to cause disorders with platelet defects including WAS and X-linked thrombocytopenia....

journal_title：International journal of molecular medicine

authors： Chatchatee P,Srichomthong C,Chewatavorn A,Shotelersuk V

更新日期：2003-12-01 00:00:00

abstract：:Thrombospondin-1 (TSP-1) is a naturally occurring anti-angiogenic compound that induces apoptosis of endothelial and cancer cells via its receptor CD36. The objectives of our study were to investigate the in vitro effects of TSP-1 on the apoptosis of primary human leukemia cells as well as leukemia cell lines and the ...

journal_title：International journal of molecular medicine

authors： Li K,Yang M,Yuen PM,Chik KW,Li CK,Shing MM,Lam HK,Fok TF

更新日期：2003-12-01 00:00:00

abstract：:Dietary fat is an important factor involved in the pathogenesis of inflammatory bowel disease (IBD). It remains unclear how n-3 and n-6 fatty acids modulate intestinal inflammation. In this study, we investigated the effects of n-3 and n-6 fatty acid-rich diets on trinitrobenzene sulfonic acid (TNBS)-induced enteritis...

journal_title：International journal of molecular medicine

authors： Andoh A,Tsujikawa T,Ishizuka I,Araki Y,Sasaki M,Koyama S,Fujiyama Y

更新日期：2003-11-01 00:00:00

abstract：:Sprouty (SPRY) was first identified in a genetic screen in Drosophila to be an antagonist of fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling, seemingly by inhibiting the Ras/MAP kinase pathway. During large scale DNA sequencing of the human fetal brain cDNA library, we cloned a novel splice ...

journal_title：International journal of molecular medicine

authors： Wang L,Ji C,Wu H,Xu J,Wu Q,Dai J,Yin G,Ye X,Gu S,Xie Y,Mao Y

更新日期：2003-11-01 00:00:00

abstract：:Van der Woude syndrome (VWS) is the most common autosomal dominant disorder with characteristic lip pits and clefts of the lip and/or palate (CL/P). The interferon regulatory factor 6 gene (IRF6) has been recently identified as the gene mutated in patients with VWS. Here, we report two novel mutations of IRF6 in two u...

journal_title：International journal of molecular medicine

authors： Kim Y,Park JY,Lee TJ,Yoo HW

更新日期：2003-10-01 00:00:00

abstract：:The fusion gene of herpes simplex virus thymidine kinase and green fluorescent protein (TK-GFP) was shown to be a versatile tool for examining the features of thymidine kinase/ganciclovir gene therapy in vitro. In this study, we used viral vectors carrying the fusion gene to characterize the aspects of this gene thera...

journal_title：International journal of molecular medicine

authors： Pasanen T,Hakkarainen T,Timonen P,Parkkinen J,Tenhunen A,Loimas S,Wahlfors J

更新日期：2003-10-01 00:00:00

abstract：:Evidence is available that exendin-4 (EX4), a glucagon-like peptide-1 receptor (GLP-1R) agonist acutely stimulates hypothalamo-pituitary-adrenal (HPA) axis in the rat. EX4 is a potent insulinotropic agent, which is currently under clinical trial for treatment of type 2 diabetes. Since diabetes is known to affect adren...

journal_title：International journal of molecular medicine

authors： Malendowicz LK,Neri G,Nussdorfer GG,Nowak KW,Zyterska A,Ziolkowska A

更新日期：2003-10-01 00:00:00

abstract：:Stannous ion, as a chloride salt, influenced on the survival and adhesive properties of two toxigenic Corynebacterium diphtheriae of the sucrose-fermenting (241 strain) and non-sucrose-fermenting (CDC-E8392 strain) biotypes. Differences in survival fractions suggested differences in susceptibility of strains to bacter...

journal_title：International journal of molecular medicine

authors： Silva De Souza SM,Hirata R Jr,Moreira LO,Gomes ML,Braga De Andrade AF,Bernardo-Filho M,Mattos-Guaraldi AL

更新日期：2003-10-01 00:00:00

abstract：:Ezrin is a key protein in membrane-cytoskeleton interaction. Expression of ezrin in actin-rich cell surfaces may play a role in the modulation of cell shape and adhesion. The aim of this study was to detect ezrin, actin and cytoskeleton and to explore their relationship in the apoptosis of esophageal epithelial cells ...

journal_title：International journal of molecular medicine

authors： Shen ZY,Xu LY,Li EM,Li JT,Chen MH,Shen J,Zeng Y

更新日期：2003-09-01 00:00:00

abstract：:Neural stem cell transplantation is a promising new treatment of ischemic or traumatic brain injury. We have now investigated the involvement of the peroxynitrite - poly(ADP-ribose) polymerase (ONOO- - PARP) activation cascade in brain trauma and neural stem cell transplantation. The forelimb motor cortex of adult mal...

journal_title：International journal of molecular medicine

authors： Lacza Z,Horváth EM,Komjáti K,Hortobágyi T,Szabó C,Busija DW

更新日期：2003-08-01 00:00:00

abstract：:Ghrelin is an endogenous ligand of the growth hormone secretagogue receptor (GHS-R), which was originally isolated from rat stomach. Ghrelin and GHS-R are also expressed in several peripheral tissues, including adrenal glands, and this prompted us to study ghrelin expression and ghrelin-binding site localization in th...

journal_title：International journal of molecular medicine

authors： Tortorella C,Macchi C,Spinazzi R,Malendowicz LK,Trejter M,Nussdorfer GG

更新日期：2003-08-01 00:00:00

abstract：:Blood supply plays a crucial role in solid tumour development and leukaemogenesis. It has been suggested that blocking of angiogenesis could be possible in cancer therapy. We have demonstrated the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various human solid tumour cancer cell lines as we...

journal_title：International journal of molecular medicine

authors： Chow LM,Chui CH,Tang JC,Lau FY,Yau MY,Cheng GY,Wong RS,Lai PB,Leung TW,Teo IT,Cheung F,Guo D,Chan AS

更新日期：2003-08-01 00:00:00

abstract：:Human gastric cancer SNU 484 cells express mutant p16, which migrates slower than the wild-type p16. We constructed an expression vector containing human p16 cDNA to evaluate the cytotoxic effects of exogenous p16 expression on SNU 484 cell proliferation and to explore the potential use of p16 in cancer gene therapy. ...

journal_title：International journal of molecular medicine

authors： Jeong YW,Kim KS,Oh JY,Park JC,Baek WK,Suh SI,Suh MH,Lee JC,Cho JW

更新日期：2003-07-01 00:00:00

abstract：:Many lines of evidence have shown that preproglucagon-derived peptides affect steroid secretion from dispersed adrenocortical cells, and that streptozotocin (STZ)-induced experimental diabetes alters adrenocortical-cell function. Hence, we compared the effects of glucagon, glucagon-like peptide (GLP)-1 and GLP-2 on ba...

journal_title：International journal of molecular medicine

authors： Malendowicz LK,Spinazzi R,Nussdorfer GG,Trejter M

更新日期：2003-07-01 00:00:00

abstract：:CREB binding protein (CBP) plays a central role in cell differentiation and proliferation, interacting with a large number of nuclear factors. To find novel nuclear factors associating with CBP, we have carried out yeast two-hybrid screening of human chondrocyte cDNA library using the C/H3 region of CBP as a bait and ...

journal_title：International journal of molecular medicine

authors： Hirose T,Fujii R,Nakamura H,Aratani S,Fujita H,Nakazawa M,Nakamura K,Nishioka K,Nakajima T

更新日期：2003-06-01 00:00:00

abstract：:Manumycin was reported to have inhibitory effect on farnesyltransferase by competing with the farnesyl pyrophosphate substrate. It exhibited different antiproliferative activity in human hepatocellular carcinoma HepG2 cells, primary cultured human cardiac muscle cells and human liver cells (CLC). HepG2 cells overexpre...

journal_title：International journal of molecular medicine

authors： Zhou JM,Zhu XF,Pan QC,Liao DF,Li ZM,Liu ZC

更新日期：2003-06-01 00:00:00

abstract：:Dextromethorphan is a widely used anti-tussive drug with non-competitive antagonistic effects on excitatory amino acid receptors of the N-methyl-D-aspartate (NMDA) type. This study examined the effect of daily dextromethorphan administration on gene expression in rat brain hippocampus and cortex regions using Rat 5K c...

journal_title：International journal of molecular medicine

authors： Lee KH,Ahn JI,Yu DH,Koh HC,Kim SH,Yang BH,Lee YS,Lee YS

更新日期：2003-05-01 00:00:00

abstract：:We analyzed a series of ovarian carcinomas from patients treated with cisplatin-based chemotherapy for loss of heterozygosity (LOH) to determine the relationship between microsatellite alteration and prognosis. Patients with tumors that had lost alleles at loci 3p14-21, 12qter, or 17p13.3 showed significantly reduced ...

journal_title：International journal of molecular medicine

authors： Nakayama S,Nakayama K,Takebayashi Y,Hata K,Fujiwaki R,Fukumoto M,Miyazaki K

更新日期：2003-05-01 00:00:00

abstract：:Resistin is thought to cause insulin resistance and link obesity to type 2 diabetes mellitus. However, little is known about the effects of neuropeptide Y (NPY) on resistin gene expression in white adipose tissue (WAT). Resistin gene expression was determined by northern blot analysis in food-deprived mice after NPY a...

journal_title：International journal of molecular medicine

authors： Yuzuriha H,Inui A,Goto K,Asakawa A,Fujimiya M,Kasuga M

更新日期：2003-05-01 00:00:00

abstract：:Heparin/heparan sulfate interacting protein (HIP) was initially identified as an adhesion molecule from a human uterine epithelial cell line. It was previously demonstrated that HIP was upregulated in human colorectal cancer. However, its expression was significantly lower in Dukes' D samples compared to earlier Dukes...

journal_title：International journal of molecular medicine

authors： Wang Y,Tan S,Hooi SC

更新日期：2003-04-01 00:00:00

abstract：:Racemic fluoxetine is a widely used SSRI antidepressant compound having also anticonvulsant effect. In addition, it was shown that it blocked several types of voltage gated ion channels including neural and cardiac calcium channels. In the present study the effects of enantiomers of fluoxetine (R(-)-fluoxetine and S(+...

journal_title：International journal of molecular medicine

authors： Magyar J,Rusznák Z,Harasztosi C,Körtvély A,Pacher P,Bányász T,Pankucsi C,Kovács L,Szûcs G,Nánási PP,Kecskeméti V

更新日期：2003-04-01 00:00:00

abstract：:Apoptosis may limit the utility of cell-based therapies for articular cartilage disorders. We tested the hypothesis that chondrocyte apoptosis can be reduced by optimizing the conditions employed to expand chondrocyte numbers in culture. Chondrocyte apoptosis was examined in monolayer and suspension culture, in the pr...

journal_title：International journal of molecular medicine

authors： Takahashi T,Yamamoto H,Ogawa Y,Yoshida S,Kataoka S,Majumdar M,Morris E,Tripple SB

更新日期：2003-03-01 00:00:00

abstract：:Although gender influences T-cell, macrophage and organ functions following trauma-hemorrhage and resuscitation (T-H), it remains unknown whether it also influences polymorphonuclear cell (PMN) activity under such conditions. To study this, proestrus female and male Sprague-Dawley rats underwent trauma-hemorrhage foll...

journal_title：International journal of molecular medicine

authors： Toth B,Schwacha MG,Kuebler JF,Bland KI,Wang P,Chaudrya IH

更新日期：2003-03-01 00:00:00

abstract：:Adrenomedullin (AM) is a hypotensive peptide, which originates from the proteolytic cleavage of pro(p)AM and acts via AM22-52-sensitive receptors. Reverse transcription polymerase chain reaction allowed the detection of the specific mRNAs of pAM in the mononuclear hematopoietic cells of the cord blood and immunocytoch...

journal_title：International journal of molecular medicine

authors： Del Pup L,Belloni AS,Carraro G,De Angeli S,Parnigotto PP,Nussdorfer GG

更新日期：2003-02-01 00:00:00

abstract：:The storage period of autologous blood of hemodialysis (HD) patients was investigated with focus on the red blood cell life span. Healthy subjects, HD patients administered recombinant human erythropoietin (rHuEPO) preparation at 4,500 units/week and at 9,000 units/week were classified into 3 groups, respectively. Blo...

journal_title：International journal of molecular medicine

authors： Tsuchida K,Nakatani T,Sugimura K,Yoshimura R,Takemoto Y

更新日期：2003-02-01 00:00:00

abstract：:Down syndrome (DS) patients, after the fourth decade of life, display some neurophatological features of the Alzheimer's disease (AD). Several hypotheses suggested that apoE4 protein, an AD risk factor, might promote amyloid formation by stabilizing an aggregated conformation of the beta amyloid protein (Abeta). This ...

journal_title：International journal of molecular medicine

authors： Folin M,Baiguera S,Conconi MT,Pati T,Grandi C,Parnigotto PP,Nussdorfer GG

更新日期：2003-02-01 00:00:00

abstract：:Idiopathic cardiomyopathy is reviewed from molecular standpoint. About a half of all patients with hypertrophic cardiomyopathy show intra-familial occurrence. In familial hypertrophic cardiomyopathy, nine gene abnormalities have been discovered in the sarcomere, i.e. the genes of beta cardiac myosin heavy chain, cardi...

journal_title：International journal of molecular medicine

authors： Takeda N

更新日期：2003-01-01 00:00:00

abstract：:O6-methylguanin-DNA methyltransferase (MGMT) is a DNA repair enzyme that transfers methyl groups from O6-methylguanine to itself. Alkylation of DNA at the O6 position of guanine is the first step by alkylating agents in inducing DNA mutations in an organism. When MGMT and the mismatch repair (MMR) system are impaired,...

journal_title：International journal of molecular medicine

authors： Kohya N,Kitajima Y,Kitahara K,Miyazaki K

更新日期：2003-01-01 00:00:00

abstract：:Microsatellite instability (MSI) and loss of heterozygosity (LOH) was investigated in paired tumour and normal tissue DNA from 108 predominantly premenopausal breast cancer patients (under age 45 years at presentation) for 25 simple repeat loci interspersed across 11 chromosomes. MSI was observed at a single locus in ...

journal_title：International journal of molecular medicine

authors： Luqmani YA,Mathew M,Lobo S

更新日期：2003-01-01 00:00:00

abstract：:Most peptidic growth factors (GFs) that act as mitogens for different cell types bind to tyrosine kinase receptors (RTKs) and trigger complex intracellular signal transduction pathways finally leading to cell proliferation. Among the early events induced by GFs, cytosolic calcium increase plays a key role, and in part...

journal_title：International journal of molecular medicine

authors： Munaron L

更新日期：2002-12-01 00:00:00

abstract：:We are interested in the possibility of a new prostate cancer therapy that would control tumor malignancy via induction of terminal cell differentiation. We previously reported that 12-O-tetra-decanoylphorbol-13-acetate (TPA) induces differentiation into cells with characteristics of microglia and decreases the malign...

journal_title：International journal of molecular medicine

authors： Suzuki T,Shimizu T,Satoh K,Sugibayashi R,Takeda K

更新日期：2002-12-01 00:00:00

abstract：:We examined the P16 expression by immunohistochemical stain and detected the methylation by methylation specific polymerase chain reaction (MSP) in 48 primary oral squamous cell carcinoma (SCC) tissues. The results showed that 20/48 (41.7%) of cancerous tissues had CpG methylation around the promoter region, but 8/48 ...

journal_title：International journal of molecular medicine

authors： Huang MJ,Yeh KT,Shih HC,Wang YF,Lin TH,Chang JY,Shih MC,Chang JG

更新日期：2002-11-01 00:00:00

abstract：:We had shown previously that the novel, marine, anticancer compound dehydrothyrsiferol (DHT) does not modulate P-glycoprotein (P-gp) dependent drug efflux. Many chemotherapeutics with clinical impact are substrates for the structurally distant related membrane transport protein MRP1 (multidrug resistance-associated pr...

journal_title：International journal of molecular medicine

authors： Pec MK,Aguirre A,Fernández JJ,Souto ML,Dorta JF,Villar J

更新日期：2002-11-01 00:00:00

abstract：:Whether a common and a single clone present, or not, among follicles of follicular lymphoma (FL) was examined in 12 cases with FL. Histologic grade was I in 6 cases, II in 3, and III in 3. DNA was selectively extracted from the neoplastic follicles of paraffin-embedded samples with use of laser capture microdissection...

journal_title：International journal of molecular medicine

authors： Yamauchi A,Nakatsuka S,Miyanaga I,Hoshida Y,Sakamoto H,Aozasa K,Osaka Lymphoma Study Group.

更新日期：2002-11-01 00:00:00

abstract：:Hirschsprung disease (HSCR), or congenital intestinal aganglionosis, is a common hereditary disorder causing intestinal obstruction, thereby showing considerable phenotypic variation in conjunction with complex inheritance. Moreover, phenotypic assessment of the disease has been complicated since a subset of the obser...

journal_title：International journal of molecular medicine

authors： Pusch CM,Sasiadek MM,Blin N

更新日期：2002-10-01 00:00:00

abstract：:Our objectives were to determine whether heme oxygenase-1 is a second messenger for prolactin-mediated angiogenesis. Endothelial cell proliferation and angiogenesis assay demonstrated that cell number and capillary formation were increased by prolactin (10 and 25 ng/ml). Both protein synthesis and mRNA analysis confir...

journal_title：International journal of molecular medicine

authors： Malaguarnera L,Pilastro MR,Quan S,Ghattas MH,Yang L,Mezentsev AV,Kushida T,Abraham NG,Kappas A

更新日期：2002-10-01 00:00:00